Shiki Toono wakes up in a hospital to discover that the walls and the people around him are covered in black lines. Unable to remember his past, Shiki is informed that he survived a fatal car accident, which left a fragment lodged in his chest. His attraction to the lines leads him to trace over an object, revealing his ability to destroy it. After realizing that only he can see these lines, Shiki is given a pair of glasses to hide them, before starting a new life with his relatives. Eight years later, the death of his father leads Shiki back to the Toono mansion, his childhood home. One day, leaving school due to anxiety, he passes a mysterious woman. A sudden urge overtakes him, and once again, he uses his ability to trace the lines that cover her. Soon after, he wakes up in the mansion, questioning whether what had transpired was a dream or reality. Shingetsutan Tsukihime tells a tale enveloped in adventure, romance, and mystery. As Shiki begins to unveil his past, what lingers in the unknown soon comes back to haunt him.