Mazinger Otome reimagines the mechas from the Mazinger franchise and the Steel Jeeg series as androids integrated into everyday society. The story follows these android girls as they navigate high school life and confront threats ranging from school delinquents to the Mechanical Beast Army. The main protagonist is Seto Magami, an android modeled after Mazinger Z, who is treated as Juzo Magami's granddaughter. Later, with the arrival of the New Mazinger Army, heated arguments and fights break out between them and the original cast.