Medusa serves as the primary and most active antagonist in Soul Eater. As Crona's mother, she manipulates her child into harvesting every soul they encounter, explicitly telling Crona that taking any desired soul is acceptable. When Crona begins to hesitate, Medusa implants a hypnotic spell in her mind, compelling her to fight and kill. To gain access to student files, Medusa disguises herself as a teacher and nurse at Shibusen, adopting a supportive persona to conceal her true nature. She remains at the school to study students and identify those capable of becoming kishin (demon gods). Medusa is prepared to exploit anyone and everyone to advance her plans by any means necessary, even planting snake bombs in the bodies of fellow witches to ensure their cooperation. She can utilize them without lowering her soul protection, which prevents others from discovering her. Her personality mirrors Dr. Stein's, as she views everyone and everything as an experiment. Her theme is snakes, reflected in her magic, clothing, and her constant chant of "Nake snake cobra cobubra." Thousands of snakes reside within her body, ready to execute her commands. She engages in an intense battle with Dr. Stein in the school's lowest level to facilitate the awakening of the first kishin. Although she initially holds the upper hand, her momentary pleasure at Asura's successful resurrection allows Stein to slice her in half. After making one final attack and claiming to love him, he skewers her through the skull, and her body evaporates. However, a snake adorned with Medusa's arrow markings is seen escaping the city through a drainpipe on the outskirts, most likely her transformed self. Medusa is subsequently proven to still be alive, having possessed a young girl named Rachel. After acquiring the legendary artifact "BREW," she leads a group of Shibusen students in an attack against her older sister, the witch Arachne.
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